Principles of Genetic Manipulation in Mice - a practical approach, 1st edition - Details

Details

Principles of Genetic Manipulation in Mice - a practical approach, 1st edition

Date: 1 - 5 April 2024

This laboratory-based training course will cover the fundamental principles and techniques of genetic manipulation of mouse embryos and ES cells for a broad range of applications, namely for creating mutant and transgenic models for biomedical research.

By end of the course the participants should:

• Have a good understanding of concepts and practices in the field of genetic manipulation in mice for purposes of basic and translational research
• Have acquired practical skills in specific experimental steps involved in the generation of genetically altered animal models
• Be familiar with current genome editing techniques
• Be able to apply the acquired knowledge in the design of genetic manipulation experiments

Programmatic content

• Principles of genetic manipulation in mice
• Overview of mouse development
• Transgenesis and mutagenesis
• Targeted manipulation of embryonic stem (ES) cells
• Recovery and culture of preimplantation-stage mouse embryos
• Micromanipulation of mouse zygotes: pronuclear injection 
• Micromanipulation of mouse morulae: ES cell injection
• Implantation: oviduct and uterine transfer
• Conditional mutagenesis
• Novel approaches for genome editing: programmable nucleases
• Mechanisms of gene editing
• CRISPR/Cas system
• Experimental design of genome alterations
• Detection and analysis of mouse genome alterations

Teaching methodology

All topics will be covered in once-daily morning lectures followed by practical sessions, which will entail the demonstration of the experimental techniques followed by hands-on execution by the participants. The learning process will develop through direct contact with the experimental protocols and hands-on work.

Evaluation type and criteria

The participants will be evaluated continuously throughout the practical sessions and by the production of an original experimental project on a related topic

Coordinator

Miguel Luz Soares, Faculty of Medicine of the University of Porto

Faculty

Miguel Luz Soares, PhD

Carlos Daniel Vasconcelos, MSc (Faculty of Sciences of the Charles University, Prague, Czechia)

Ana Filipa Dias, MSc (Institute of Biomedical Sciences Abel Salazar, University of Porto)

Target audience

Undergraduates, graduate students, researchers and faculty in the areas of Biological or Medical Sciences.

Admission criteria

Attendance of higher education or university training in Biological and Medical Sciences

Dates

1 - 5 April 2024

Schedule

Monday to Friday - 09h00 to 18h00

Admission

Applications / registration will be conducted online only, on the website of the Faculty of Medicine of Porto.     

Online application

1 - 28 February 2024

Online registration

18 - 24 March 2024

Number of participants

12 (max) / 6 (min).

Ranking criteria

Curricular analysis, order of application. Eligibility according to the admission criteria.

Credits

3 ECTS

Tuition fee

350 €

Language

English